Anti-Renin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P00797

Core Product Specifications and Parameters

Biological Background: Renin Function and Localization

• Renin is a highly specific aspartyl protease encoded by the REN gene; it is synthesized as a preproprotein with a signal peptide and activated by cleavage at basic residues.
• Its only known function is to cleave angiotensinogen to produce angiotensin I, the rate-limiting step of the renin-angiotensin system, which regulates blood pressure and electrolyte balance.
• Renin is secreted into the circulation, but it can also associate with membranes; it is classified as a secreted and membrane protein.
• Post-translational modifications include N-linked glycosylation and intrachain disulfide bonds that are critical for proper folding and activity.
• Keywords also highlight that REN is associated with disease variants, including those linked to renal tubular dysgenesis (RTD), hyperuricemic nephropathy (HNFJ2), and autosomal dominant tubulointerstitial kidney disease (ADTKD4), consistent with its aliases.
• The molecular weight of mature renin is approximately 45 kDa, consistent with the calculated mass of 45,057 Da from UniProt.

Experimental Guidance and Technical Tips

• For Western blot (WB), the antibody may detect bands near 45 kDa in kidney lysates; consider using a positive control such as recombinant renin protein.
• In immunohistochemistry (IHC-P), evaluate antigen retrieval methods on paraffin-embedded kidney tissue sections to optimize signal.
• For ELISA applications, pair this antibody with a suitable monoclonal antibody to develop a sandwich ELISA for renin quantification.
• Given the immunogen region (aa 250–406), the antibody likely recognizes both prorenin and active renin; confirm specificity in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers a reliable supply of high-quality antibodies, including this anti-renin polyclonal antibody, along with complementary reagents such as recombinant proteins and detection systems. Our integrated sourcing platform streamlines the procurement of validated antibody pairs, bulk ancillary materials, and rare-target raw materials to accelerate assay development for hypertension and renal function testing.