Anti-Renin Monoclonal Antibody for WB, IF/ICC, ELISA - P00797

Core Product Specifications and Parameters

Biological Background: Renin Function and Localization

• Renin is a highly specific aspartyl protease that catalyzes the cleavage of angiotensinogen to angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS).
• It plays a central role in blood pressure regulation and electrolyte homeostasis by stimulating aldosterone secretion and promoting sodium reabsorption in the kidney.
• The protein is synthesized as a preprorenin, processed to prorenin, and activated by proteolytic removal of the 43-amino acid prosegment to yield mature renin.
• Renin is primarily secreted into the bloodstream but can also be found associated with cellular membranes.
• As a member of the aspartyl protease family, its activity depends on two catalytic aspartic acid residues within a conserved active site.
• The enzyme is glycosylated and contains disulfide bonds that contribute to its structural stability and proper folding.
• Alternative splicing generates multiple transcript variants, potentially giving rise to different protein isoforms.
• Mutations in the REN gene are implicated in inherited renal disorders such as renal tubular dysgenesis and hyperuricemic nephropathy.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 250–406, a region that is highly conserved across human, mouse, and rat, supporting cross-reactivity in these species.
• For Western blotting, the predicted molecular weight is approximately 45 kDa; consider using appropriate gel percentages and transfer conditions.
• In immunofluorescence, signal may localize to secretory vesicles or the plasma membrane; include appropriate permeabilization steps and validate in relevant cell lines.
• For ELISA, employ paired antibody strategies and titrate the antibody to optimize signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-Renin product for cardiovascular and renal disease targets. Beyond antibodies, we offer a comprehensive portfolio of ancillary reagents, bulk supply options, and rare-target sourcing to support your entire development pipeline. Let CamelBio streamline your procurement and accelerate your project from concept to clinic.