Anti-RELN Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P78509

Core Product Specifications and Parameters

Biological Background: RELN Function and Localization

• Reelin (RELN) is a large extracellular matrix serine protease secreted by pioneer neurons, playing a critical role in neuronal layering of the cerebral cortex and cerebellum during neurodevelopment.
• It coordinates cell positioning and migration by regulating microtubule function in neurons and acting as a barrier to migration for sympathetic preganglionic neurons in the spinal cord.
• Reelin binds to lipoprotein receptors VLDLR and LRP8/APOER2, inducing tyrosine phosphorylation of the adaptor protein DAB1 and modulating TAU phosphorylation, a key pathway in neuronal signaling.
• Its serine protease activity is implicated in the modulation of cell adhesion, contributing to tissue architecture.
• Reelin is localized to the extracellular space and extracellular matrix, consistent with its role in intercellular signaling.
• In the developing brain, it is abundantly produced by Cajal-Retzius cells and cerebellar granule cells; in adults, it is expressed in GABAergic interneurons of the prefrontal cortex, temporal cortex, hippocampus, and glutamatergic granule cells of the cerebellum.
• Reelin expression is also detected in fetal and adult liver, and its levels are reduced by approximately 50% in patients with schizophrenia, linking it to neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50-190 of human RELN, targeting the N-terminal region. For Western blotting, note that the full-length protein is approximately 388 kDa; consider using low-percentage acrylamide gels and extended transfer times to ensure efficient detection.
• For immunohistochemistry (IHC-P), optimize antigen retrieval methods as recommended for large extracellular matrix proteins, and validate staining specificity with appropriate controls.
• In ELISA applications, this polyclonal antibody may be suitable for both capture and detection, but cross-reactivity with other species should be confirmed in the intended sample matrix.
• Given the high molecular weight and potential for proteolytic processing, verify band patterns in relevant tissue lysates and consider using protease inhibitors during sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive portfolio of IVD raw materials, with this RELN antibody suitable for neuroscience and developmental disease research. Our supply capabilities extend to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, ensuring reliable access to critical components throughout your assay development pipeline.