Anti-RELB Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q01201

Core Product Specifications and Parameters

Biological Background: RELB Function and Localization

• RELB, also known as I-Rel, is a member of the NF-kappa-B transcription factor family, characterized by a conserved Rel homology domain responsible for DNA binding, dimerization, and nuclear localization.
• NF-kappa-B complexes are homo- or heterodimers formed by RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL, and NFKB2/p52. RELB forms transcriptionally active heterodimers with p50 or p52 but does not associate with RELA/p65 or REL.
• As a core component of NF-kappa-B signaling, RELB is involved in diverse biological processes, including inflammation, immunity, cell differentiation, growth, tumorigenesis, and apoptosis, by promoting target gene expression at kappa-B DNA sites.
• RELB localizes predominantly to the nucleus and cytoplasm, with additional associations with the cytoskeleton, microtubule-organizing center, and centrosome, suggesting roles beyond transcriptional regulation.
• The protein undergoes post-translational modifications such as phosphorylation and methylation, which modulate its activity, stability, and interactions with inhibitory I-kappa-B proteins.
• RELB is essential for T and B lymphocyte maturation and function, highlighting its critical role in adaptive immunity. Related references: PMID:26385063
• RELB regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer in a CRY1/CRY2-independent manner, with enhanced repression in the presence of NFKB2/p52.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of RELB is ~62 kDa; consider using appropriate positive control lysates from human, mouse, or rat cells.
• For immunohistochemistry (IHC-P), antigen retrieval optimization may be necessary; include relevant tissue controls and validate staining specificity.
• In ELISA applications, titrate both coating antigen and antibody to determine optimal working concentrations; the immunogen region (aa 296-436) may be used for assay design.
• As a polyclonal antibody, it may detect multiple epitopes; batch-to-batch consistency should be verified in the intended application.
• Cross-reactivity with mouse and rat allows for translational studies, but antibody performance should be validated in the corresponding species’ sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-RELB antibody supports NF-kappa-B pathway research and can be integrated into diagnostic assay development processes. Leverage our comprehensive support from concept to clinic to streamline your IVD projects.