Anti-RELB Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q01201

Core Product Specifications and Parameters

Biological Background: RELB Function and Localization

• RELB (transcription factor RelB, also known as I-Rel) is a member of the NF-κB family of transcription factors, encoded by the RELB gene in humans. It functions as a DNA-binding activator or repressor in homo- or heterodimeric complexes.
• NF-κB dimers containing RELB (e.g., RelB-p50, RelB-p52) bind to κB sites in target gene promoters, playing critical roles in inflammation, immunity, cell growth, differentiation, and apoptosis.
• RELB is required for both T and B lymphocyte maturation and function. Related references: PMID:26385063
• It contributes to pancreatic ductal adenocarcinoma resistance by participating in the NUPR1/RELB/IER3 survival pathway under cell stress conditions.
• RELB regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer, independently of CRY proteins, with enhanced repression in the presence of NFKB2/p52.
• The protein undergoes post-translational modifications including phosphorylation and methylation, and its activity is regulated by cytoplasmic sequestration via IκB inhibitors and subsequent release following IKK-mediated degradation.
• Subcellular localization includes the nucleus, cytoplasm, and centrosome (microtubule organizing center), consistent with its dynamic shuttling in NF-κB signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 296–436 of human RELB (NP_006500.2), covering a C-terminal region. This design suggests the antibody may recognize full-length RELB and potentially its major isoforms. Consider validating specificity in the relevant species and sample types.
• For Western blotting, a band around 62 kDa is expected; additional bands from post-translational modifications or degradation may appear. Use appropriate positive and negative controls.
• In immunohistochemistry (IHC-P), nuclear and cytoplasmic staining patterns may be observed depending on NF-κB activation status. Optimize antigen retrieval and detection protocols.
• For immunoprecipitation (IP) and ELISA applications, ensure antibody compatibility with native or denatured protein conformations as required.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw material supplier, CamelBio provides diagnostic manufacturers, labs, and research institutes with reliable access to monoclonal antibodies like this anti-RELB clone. Our comprehensive offerings include validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. We support your NF-κB pathway and transcription factor research projects from concept to clinical application.