Anti-REG3A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q06141

Core Product Specifications and Parameters

Biological Background: REG3A Function and Localization

• REG3A (Regenerating islet-derived protein 3-alpha), also known as hepatointestinal pancreatic protein (HIP/PAP) or pancreatitis-associated protein 1, is a bactericidal C-type lectin.
• It acts exclusively against Gram-positive bacteria by binding to surface-exposed carbohydrate moieties of peptidoglycan and killing bacteria by forming a hexameric membrane-permeabilizing oligomeric pore (PubMed:16931762, PubMed:24256734).
  Related references: PMID:16931762, PMID:24256734
• REG3A functions as a hormone, activating the EXTL3 receptor to trigger cell-specific signaling pathways (PubMed:19158046, PubMed:22727489, PubMed:27830702).
  Related references: PMID:19158046, PMID:22727489, PMID:27830702
• It regulates keratinocyte proliferation and differentiation after skin injury via the EXTL3-PI3K-AKT pathway and inhibits skin inflammation by suppressing IL-6 and TNF (PubMed:22727489, PubMed:27830702).
  Related references: PMID:22727489, PMID:27830702
• In the pancreas, REG3A permeabilizes beta-cell membranes and stimulates their proliferation (PubMed:19158046).
  Related references: PMID:19158046
• The protein is secreted and contains disulfide bonds typical of the C-type lectin family.
• Tissue expression is constitutive in the intestine, low in healthy pancreas, but highly upregulated in psoriasis keratinocytes and during acute pancreatitis (PubMed:22727489, PubMed:1469087).
  Related references: PMID:22727489, PMID:1469087
• REG3A also exhibits bacteriostatic activity.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 19 kDa. Include appropriate positive and negative controls, and validate band specificity in your target tissue or cell line.
• In immunohistochemistry (IHC-P), antigen retrieval may be required. Test on known positive tissues such as inflamed pancreas or psoriatic skin.
• For ELISA, consider pairing with a detection antibody specific to REG3A; optimize coating concentrations for your assay format.
• Due to the polyclonal nature, some lot-to-lot variation may occur; we recommend titrating each new lot and including internal reference standards.
• Cross-reactivity with mouse and rat is indicated; however, we suggest verifying species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories. This REG3A antibody supports antimicrobial and inflammatory disease research, and CamelBio can also assist in sourcing rare-target raw materials and developing customized immunoassay components. Contact us to streamline your R&D and production pipeline.