Anti-REEP5 Polyclonal Antibody for WB, ELISA - Q00765

Core Product Specifications and Parameters

Biological Background: REEP5 Function and Localization

• Receptor expression-enhancing protein 5 (REEP5), also known as DP1, TB2, or polyposis locus protein 1, is a member of the DP1/YOP1 family.
• Encoded by the REEP5 gene (C5orf18), this protein undergoes alternative splicing, producing multiple isoforms.
• REEP5 localizes to the endoplasmic reticulum and sarcoplasmic reticulum membranes, where it functions as a multi-pass transmembrane protein.
• In cardiomyocytes, REEP5 is critical for the organization and function of the sarcoplasmic reticulum, playing an essential role in heart development and cardiac function.
• Tissue expression is prominent in the heart at the protein level, with additional expression detected in circumvallate papillae and testis.
• The protein has a predicted molecular weight of approximately 21 kDa (UniProt: 21493 Da).

Experimental Guidance and Technical Tips

• For Western blot (WB), optimize the primary antibody dilution; a starting range of 1:500–1:2000 is commonly used for polyclonal antibodies.
• Given the predicted molecular weight of REEP5 (~21 kDa), a 12–15% SDS-PAGE gel is recommended for optimal separation.
• The antibody detects REEP5 in human, mouse, and rat; include species-matched positive controls to confirm reactivity.
• In ELISA, use the antibody as either capture or detection reagent and titrate to establish the optimal working concentration.
• Store the antibody at -20°C upon receipt and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material sourcing, connecting diagnostic manufacturers and researchers with high-quality reagents. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-REEP5 product, and bulk ancillary reagents—all tailored to support cardiac and sarcoplasmic reticulum research. With a commitment to covering every stage from concept to clinic, CamelBio streamlines your supply chain for reliable assay development.