Anti-REEP2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9BRK0

Core Product Specifications and Parameters

Biological Background: REEP2 Function and Localization

• REEP2 (Receptor expression-enhancing protein 2) is an endoplasmic reticulum (ER) membrane protein essential for ER network formation, shaping, and remodeling. It may also enhance cell surface expression of odorant receptors.
• The REEP2 gene (aliases C5orf19, SGC32445) maps to chromosome 5 and produces multiple isoforms through alternative splicing.
• Subcellular localization: integral membrane protein, predominantly located in the ER.
• Tissue specificity: highly expressed in brain, heart, and skeletal muscle; detected at lower levels in placenta, kidney, and pancreas. Also expressed in circumvallate papillae. Related references: PMID:11161817 PMID:16720576
• Post-translational modifications: classified as a phosphoprotein; phosphorylation sites have been identified by proteomics.
• Disease association: mutations in REEP2 are linked to hereditary spastic paraplegia type 72 (SPG72), a neurodegenerative disorder.
• Molecular weight: approximately 28 kDa (calculated from sequence: 28,261 Da).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 93–252 of human REEP2, which includes the C-terminal region and likely the transmembrane domain. This design favors detection of both full-length and truncated isoforms.
• For Western blot (WB), an approximately 28 kDa band is expected. Validate specificity using positive control lysates (e.g., human brain or heart) and consider knockdown/knockout controls.
• For immunofluorescence on fixed samples (IF-P), REEP2 localizes to the ER network; co-staining with an ER marker (e.g., calreticulin or PDI) is recommended.
• For ELISA, the antibody can detect recombinant REEP2 protein. Titration using the immunogen as coating antigen may help optimize conditions.
• Cross-reactivity with mouse and rat has been observed; verify sequence homology and expression levels in your experimental system before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality IVD raw materials to diagnostic manufacturers and research institutes worldwide. This anti-REEP2 polyclonal antibody supports neuroscience and cell biology studies, especially those involving ER dynamics and hereditary spastic paraplegia. Leveraging a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents, CamelBio enables seamless procurement for assay development and biomarker research.