Anti-RECQL4 Rabbit Polyclonal Antibody for WB and ELISA - O94761

Core Product Specifications and Parameters

Biological Background: RECQL4 Function and Localization

• RECQL4 is the ATP-dependent DNA helicase Q4, belonging to the RecQ helicase family.
• Functions as a DNA helicase that unwinds dsDNA with a 3'-overhang in a 3'-5' direction, capable of unwinding less than 18 base pairs.
  Related references: PMID:28653661
• May play a role in modulating chromosome segregation.
• The N-terminal domain (residues 1-54) has a preference for binding Y-shaped DNA over single- or double-stranded DNA, while the core helicase domain binds ssDNA.
  Related references: PMID:22730300, PMID:28653661
• Localizes to both the nucleus and cytoplasm.
• Ubiquitously expressed with elevated expression in thymus and testis.
• Contains key structural and functional motifs including ATP-binding, DNA-binding, helicase, hydrolase, isomerase, zinc-binding, nucleotide-binding, and phosphoprotein domains.
• Associated with several human diseases such as Rothmund-Thomson syndrome (RTS), cataracts, craniosynostosis, dwarfism, ectodermal dysplasia, and hypotrichosis.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 231–330 within the helicase domain; validate antibody specificity in your target sample using appropriate positive and negative controls.
• For Western blot, note the predicted molecular weight of ~133 kDa; use a suitable gel percentage (e.g., 6–8% SDS-PAGE) and extended transfer times to ensure efficient blotting of high-molecular-weight proteins.
• In ELISA, titrate the antibody and optimize coating buffer and blocking conditions for best signal-to-noise ratio.
• The antibody shows cross-reactivity with mouse, allowing its use in mouse tissue or cell line studies; verify performance in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for diagnostic manufacturers and research labs. In addition to high-quality antibodies like this anti-RECQL4 polyclonal antibody, we provide validated antibody pairs, recombinant proteins, and ancillary reagents to support your DNA repair and genomic stability research. Our team can assist in sourcing rare-target raw materials to accelerate your assay development.