Anti-Recoverin Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P35243

Core Product Specifications and Parameters

Biological Background: Recoverin Function and Localization

• Recoverin (RCVRN, also known as RCV1 or Protein CAR) is a 23 kDa calcium-binding protein belonging to the neuronal calcium sensor (NCS) family.
• It acts as a calcium sensor in phototransduction, regulating cone and rod photoreceptor cells by modulating rhodopsin phosphorylation in response to calcium levels.
• In low light, elevated calcium promotes recoverin binding to rhodopsin kinase (GRK1), inhibiting rhodopsin phosphorylation and prolonging light sensitivity.
• Recoverin enhances scotopic vision by improving signal transfer between rod photoreceptors and bipolar cells, and it mediates motion detection in bright light.
• It is predominantly expressed in the retina and pineal gland, with localization to photoreceptor inner and outer segments, cilia, and perikaryon.
• The protein undergoes myristoylation, facilitating calcium-dependent membrane association, and contains functional calcium-binding motifs.
• Recoverin is implicated in cancer-associated retinopathy (CAR), where autoantibodies target the protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human Recoverin (aa 1-200), suggesting the antibody may recognize the native conformation.
• For western blot (WB), a distinct band near 23 kDa is anticipated; denaturing and reducing conditions are typically employed.
• In immunohistochemistry (paraffin-embedded IHC-P), test on retina or pineal gland tissue sections; antigen retrieval may be required.
• Cross-reactivity with mouse and rat is specified; however, perform species-specific validation due to potential sequence variations.
• For ELISA applications, consider pairing with a different clone or using the antibody as a detection reagent after confirming binding activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, supporting diagnostic developers and researchers with high-quality antibodies and reagents. As a trusted partner, we supply optimized anti-Recoverin monoclonal antibodies, including validated pairs and ancillary reagents, streamlining your vision research and assay development. Our expertise extends to rare target sourcing, ensuring efficient procurement to accelerate your projects from concept to clinic.