Anti-Receptor-type tyrosine-protein phosphatase epsilon (PTPRE) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P23469

Core Product Specifications and Parameters

Biological Background: PTPRE Function and Localization

• PTPRE encodes receptor-type tyrosine-protein phosphatase epsilon, a transmembrane phosphatase belonging to the protein tyrosine phosphatase (PTP) family. Alternative promoter usage and alternative initiation produce distinct isoforms.
• Isoform 1 participates in signaling transduction pathways and phosphoprotein network topology in red blood cells, and may contribute to osteoclast formation and function.
• Isoform 2 functions as a negative regulator of insulin receptor (IR) signaling in skeletal muscle, modulating IR and IRS-1 tyrosine phosphorylation, and downstream glucose uptake.
• Both isoform 1 and isoform 2 negatively regulate FcεRI-mediated signal transduction, likely by acting on SYK to influence SLP76/LAT phosphorylation and calcium mobilization.
• Subcellular localization includes the cell membrane and cytoplasm, consistent with its transmembrane topology and catalytic domain release after proteolytic cleavage.
• The protein is subject to post-translational modifications, including glycosylation and phosphorylation, as indicated by UniProt keywords.
• Tissue expression is noted in giant cell tumor (osteoclastoma), with roles in osteoclast biology and immune cell signaling.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 20-200) that may be present in multiple isoforms; verify isoform specificity using positive and negative controls.
• For Western blotting, consider sample preparation under reducing and non-reducing conditions to detect potential cleavage products or oligomers.
• In IHC-P, a standard antigen retrieval protocol using citrate buffer (pH 6.0) may improve epitope exposure; optimize primary antibody concentration.
• For ELISA, preliminary checkerboard titrations are recommended to determine the optimal coating antigen and antibody concentration for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined procurement pathway for IVD raw materials. This PTPRE antibody supports targeted research in signal transduction, insulin signaling, and immune regulation. Our portfolio covers validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing to accelerate your assay development from early R&D to clinical application.