Anti-RDM1 (RAD52B) Polyclonal Antibody for WB, ELISA - Q8NG50

Core Product Specifications and Parameters

Biological Background: RDM1 Function and Localization

• RDM1 (RAD52 motif-containing protein 1, also known as RAD52B) is a human protein encoded by the RDM1 gene (GeneID: 201299). UniProt: Q8NG50.
• It belongs to the RAD52 family and acts as a DNA and RNA-binding protein, potentially playing a role in nucleic acid metabolism and repair.
• RDM1 may confer resistance to the chemotherapeutic agent cisplatin, suggesting involvement in cellular response to DNA damage.
• Subcellularly, RDM1 localizes to the nucleus, cytoplasm, nucleolus, PML bodies, and Cajal bodies, indicating dynamic shuttling and diverse cellular functions.
• The protein is predominantly expressed in testis, hinting at a specialized role in germ cell biology.
• Post-translational regulation is indicated by alternative promoter usage and alternative splicing, as annotated in UniProt.
• RDM1 has been identified through proteomic approaches and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region of RDM1 (aa 125-284), which may allow recognition of full-length RDM1 and potentially splice variants containing this region.
• For Western blotting, consider using whole-cell lysates or nuclear extracts to detect RDM1 given its nuclear localization, though cytoplasmic fractions may also contain protein.
• Given cross-reactivity with mouse, this antibody can be used to study RDM1 in mouse models; however, validate reactivity in the specific mouse strain and tissue of interest.
• Since RDM1 expression is enriched in testis, testis lysates may serve as a positive control for antibody validation.
• For ELISA applications, use the recombinant protein or peptide as coating antigen, and confirm antibody specificity with blocking and competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-RDM1 polyclonal antibody for DNA damage and cancer research. We offer one-stop access to antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting every stage from concept to clinical application.