Anti-RDH11 Polyclonal Antibody for WB and ELISA - Q8TC12

Core Product Specifications and Parameters

Biological Background: Retinol Dehydrogenase 11 Function and Localization

• Retinol dehydrogenase 11 (RDH11) is a member of the short-chain dehydrogenase/reductase (SDR) family, specifically the SDR7C1 subfamily, encoded by the RDH11 gene in humans.
• It catalyzes the oxidation of retinol to retinal, displaying high activity towards 9-cis, 11-cis, and all-trans-retinol, with lower activity on 13-cis-retinol, using NADP as cofactor. Related references: PMID:12036956, PMID:12226107, PMID:29410696
• RDH11 exhibits low reductive activity towards unsaturated medium-chain aldehydes such as cis-6-nonenal, but no activity toward nonanal or 4-hydroxy-nonenal. Related references: PMID:15865448
• The enzyme shows no dehydrogenase activity toward steroid substrates. Related references: PMID:12036956, PMID:12226107
• Subcellular localization is at the endoplasmic reticulum membrane, consistent with its role in lipid metabolism.
• Tissue expression is predominantly in prostate epithelial cells (both basal and luminal), with low levels in spleen, thymus, testis, ovary, small intestine, colon, peripheral blood leukocytes, kidney, adrenal gland, and fetal liver; it is not detected in prostatic stromal cells, endothelial cells, or infiltrating lymphocytes.
• UniProt keywords highlight its involvement in processes such as acetylation, alternative splicing, cataract, dwarfism, lipid metabolism, NADP utilization, and oxidoreductase activity, indicating potential disease associations and post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70-145 of human RDH11, a region potentially conserved across species, contributing to the observed cross-reactivity with rat. For Western blotting, expect a band near 35 kDa. Consider optimizing antibody dilution and sample preparation based on your specific experimental system.
• For ELISA applications, use purified recombinant RDH11 or native protein from cell lysates. Due to high prostate expression, prostate-derived cell lines (e.g., LNCaP, PC-3) or tissue homogenates are suitable positive controls.
• Validate antibody performance in the relevant sample matrix to ensure specificity, especially given the low expression levels in tissues other than prostate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, technical services, and consulting to accelerate diagnostic development from concept to clinic. For research on retinoid metabolism and prostate biology, we supply anti-RDH11 antibodies and related reagents, alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. Let us support your next project with reliable and scalable raw-material solutions.