Anti-RCVRN Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P35243

Core Product Specifications and Parameters

Biological Background: RCVRN Function and Localization

• RCVRN encodes recoverin, also known as cancer-associated retinopathy protein (CAR protein), a member of the neuronal calcium sensor family involved in visual transduction.
• Acts as a calcium sensor, regulating phototransduction in cone and rod photoreceptor cells by modulating light sensitivity in dark and dim conditions.
• In low light, high Ca²⁺ levels promote recoverin binding to and inhibition of GRK1, thereby prolonging rhodopsin (RHO) activation and enhancing signal transfer in rod photoreceptors.
• Plays a critical role in scotopic vision, improving rod photoreceptor sensitivity and facilitating detection of change and motion in bright light.
• Localizes predominantly to photoreceptor inner and outer segments, the outer segment membrane, and the perikaryon, consistent with its role in visual signal processing.
• Tissue expression is restricted to the retina and pineal gland, underscoring its specialized function in light perception.
• Post-translational modifications include myristoylation and disulfide bond formation, with calcium binding influencing its membrane association and activity.
• Classified under keywords such as calcium, lipoprotein, myristate, metal-binding, and sensory transduction, reflecting its functional attributes.

Experimental Guidance and Technical Tips

• For Western blotting, a specific band near 23 kDa is anticipated; inclusion of retina or pineal gland tissue lysates as positive controls can help confirm reactivity.
• In immunostaining applications (IF-P, IHC-P), expect predominant labeling of photoreceptor inner and outer segments; appropriate fixation and permeabilization protocols should be optimized for target accessibility.
• Given that recoverin expression is restricted to retina and pineal gland, cross-absorption or tissue-specific controls may be necessary to minimize background in other tissues.
• The immunogen comprises full-length human recoverin (aa 1-200), suggesting that the antibody may recognize both calcium-bound and calcium-free conformations; however, calcium-dependent epitopes should be investigated if conformation-specific detection is required.
• As a polyclonal antibody, lot-to-lot consistency should be verified; consider performing a titration series for each new lot to determine optimal working dilutions for your specific assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focused on visual transduction and ophthalmic disease biomarkers, we offer validated polyclonal and monoclonal antibodies, recombinant proteins, and custom development support to accelerate your assay development. Our supply chain ensures batch-to-batch consistency and scalability, bridging the gap from discovery to clinical application.