Anti-RCN2 (Reticulocalbin-2) Polyclonal Antibody for WB and ELISA - Q14257

Core Product Specifications and Parameters

Biological Background: Reticulocalbin-2 Function and Localization

• RCN2, also known as reticulocalbin-2, ERC-55, or E6BP, is a calcium-binding protein encoded by the RCN2 gene.
• The precise biological function of RCN2 remains unknown; it binds calcium ions, likely playing a role in calcium-dependent processes within the secretory pathway.
• The protein localizes to the endoplasmic reticulum lumen, consistent with its signal peptide and ER-retention motifs.
• RCN2 is ubiquitously expressed across human tissues, suggesting a housekeeping role in cellular calcium homeostasis.
• Post-translational modifications include phosphorylation (evidenced by the 'Phosphoprotein' keyword), which may regulate its activity or interactions.
• The protein contains EF-hand calcium-binding repeats and an N-terminal signal peptide, classifying it as a member of the CREC family.
• The canonical isoform has a molecular weight of approximately 37 kDa (calculated from sequence: 36,876 Da).
• Alternative splicing generates multiple transcript variants, potentially producing isoforms with distinct properties.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–180 of human RCN2, covering the N-terminal region. This polyclonal antibody is expected to recognize multiple epitopes, suitable for detecting both denatured and native protein.
• For Western blot, anticipate a specific band around 37 kDa. Include appropriate positive and negative controls, and validate in the relevant species (human, mouse, rat) cell or tissue lysates.
• In ELISA, the polyclonal nature may provide high sensitivity through signal amplification; consider pairing with a monoclonal capture antibody for sandwich assay development.
• Given the ER luminal localization, use reducing sample preparation to expose epitopes, and include a protein translocation blocker (e.g., Brefeldin A) if detecting newly synthesized protein.
• Cross-reactivity with mouse and rat has been demonstrated, enabling translational studies across these model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering this RCN2 polyclonal antibody along with complementary reagents such as recombinant antigens, blocking buffers, and detector antibodies. We provide validated antibody pairs, optimized mono- and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. Whether your research focuses on calcium signaling, protein secretion, or ER stress, our sourcing network delivers rare-target raw materials with reliable supply chains.