Anti-RCN1 Rabbit Polyclonal Antibody for WB/IF/ICC/ELISA - Q15293

Core Product Specifications and Parameters

Biological Background: Reticulocalbin-1 Function and Localization

• Reticulocalbin-1 (RCN1), also known as RCN, RCAL, PIG20, or HEL-S-84, is a calcium-binding protein primarily located in the endoplasmic reticulum lumen.
• It may regulate calcium-dependent activities within the ER or post-ER compartments, contributing to calcium homeostasis and signaling.
• Encoded by the RCN1 gene (GeneID: 5954 in humans), the protein belongs to the CREC (Cab45/reticulocalbin/ERCa45/calumenin) family of EF-hand calcium-binding proteins.
• RCN1 undergoes alternative splicing, producing multiple isoforms that may differ in localization or function.
• The protein features a signal peptide for ER targeting, repeat domains, and undergoes post-translational modifications including glycosylation and phosphorylation.
• Its metal-binding properties and presence in the ER lumen suggest involvement in protein folding, sorting, or secretory pathway regulation.
• Although ubiquitously expressed, functional studies often focus on its role in ER stress response and cellular calcium signaling.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–331 of human RCN1, excluding the signal peptide; this design may enhance detection of the mature, processed protein.
• For Western blotting, RCN1 typically migrates near 39 kDa; use whole‑cell lysates from human or mouse cells as positive controls.
• In immunofluorescence/ICC, optimize permeabilization protocols to visualize ER‑localized RCN1; co‑staining with an ER marker is recommended.
• ELISA performance should be verified using recombinant RCN1 or known positive samples; consider pairing with a detection antibody for sandwich assays.
• As with any polyclonal antibody, lot‑to‑lot consistency should be monitored; validate in your experimental system before large‑scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your research on ER calcium‑binding proteins like reticulocalbin‑1 by providing reliable IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Whether you need off‑the‑shelf products or assistance sourcing rare targets, our integrated supply chain and technical consulting streamline your journey from concept to clinic. Contact us to explore how we can accelerate your next diagnostic or life‑science project.