Anti-RCL1 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y2P8

Core Product Specifications and Parameters

Biological Background: RCL1 Function and Localization

• RCL1 (RNA 3'-terminal phosphate cyclase-like protein) is a nucleolar protein that shares sequence similarity with RNA 3'-terminal phosphate cyclases but lacks cyclase activity.
• It functions as a component of the small subunit (SSU) processome, a large ribonucleoprotein complex essential for 40S ribosomal subunit biogenesis.
• Within the SSU processome, RCL1 is involved in early pre-rRNA processing steps at cleavage sites A0, A1, and A2, which are critical for proper maturation of 18S rRNA.
• RCL1 also acts as a guanine nucleotide exchange factor (GEF) for the GTPase BMS1, promoting GDP/GTP exchange to regulate ribosome assembly.
• The protein is localized primarily in the nucleolus, consistent with its role in ribosomal RNA processing.
• Alternative splicing of RCL1 generates multiple transcript variants, potentially contributing to functional diversity.
• Post-translational modifications and proteomics identification have been reported, supporting its characterization as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 1-300 of human RCL1; for species cross-reactivity, consider verifying sequence homology in mouse and rat.
• This antibody is suitable for Western blot (WB) and ELISA applications; optimize antibody dilution and detection conditions based on your experimental setup.
• Given its predicted molecular weight of approximately 41 kDa, include appropriate molecular weight markers and validate specificity in relevant cell or tissue lysates.
• For ribosomal biogenesis studies, consider combining this antibody with other markers of nucleolar function to confirm localization or complex association.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop supply of high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like RCL1 involved in ribosomal biogenesis, CamelBio offers customized sourcing solutions and technical consultative support to accelerate assay development from concept to clinic. Partner with CamelBio to streamline your procurement and ensure consistent reagent performance for your diagnostic workflows.