Anti-RCE1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y256

Core Product Specifications and Parameters

Biological Background: RCE1 Function and Localization

• RCE1 (CAAX prenyl protease 2), also known as FACE-2 or hRCE1, is a transmembrane protease localized to the endoplasmic reticulum membrane.
• It specifically cleaves the C-terminal tripeptide from prenylated proteins ending with a CAAX motif, where C is the prenylcysteine.
• RCE1 processes a range of important signaling molecules, including K-Ras, N-Ras, H-Ras, RAP1B, and G-protein gamma-1 subunits.
  Related references: PMID:10085068
• The protein is ubiquitously expressed across human tissues, indicating a housekeeping role in post-translational modification.
• RCE1 is subject to acetylation and ubiquitin-like conjugation, and it contains multiple transmembrane helices.
• The canonical isoform has a molecular weight of approximately 36 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa 100–200) of human RCE1; cross-reactivity with mouse and rat orthologs has been reported.
• This polyclonal antibody can be used in multiple applications, including western blotting (detecting a band at ~36 kDa), immunohistochemistry (paraffin), immunofluorescence, and ELISA.
• For western blotting, consider using membrane-enriched lysates and confirm specificity by siRNA knockdown or knockout controls.
• Optimize antibody dilution for each application and sample type; typical starting concentrations can be determined in the end-user’s laboratory.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with comprehensive access to IVD raw materials, including validated polyclonal antibodies like this anti-RCE1 antibody. Whether you require ready-to-use antibody pairs, bulk reagent supply, or custom monoclonal development for rare targets such as prenyl proteases, our one-stop sourcing platform bridges your discovery-to-clinic journey efficiently.