Anti-RCC2 Rabbit Polyclonal Antibody for WB, ELISA - Q9P258

Core Product Specifications and Parameters

Biological Background: RCC2 Function and Localization

• Regulates the activity of small GTPases RAC1 and RALA, functioning as a guanine nucleotide exchange factor (GEF) for RALA and interfering with RAC1 activation (PubMed:12919680, PubMed:25074804, PubMed:26158537). Related references: PMID:12919680 PMID:25074804 PMID:26158537

• Essential for normal cell cycle progression during interphase and mitosis, ensuring accurate chromosome segregation and kinetochore-microtubule attachments (PubMed:12919680, PubMed:23388455, PubMed:26158537). Related references: PMID:12919680 PMID:23388455 PMID:26158537

• Required for normal centromere function, maintaining appropriate levels of MAD2L1, AURKB and BIRC5 at inner centromeres to ensure proper kinetochore-spindle attachment (PubMed:12919680, PubMed:26158537). Related references: PMID:12919680 PMID:26158537

• Critical for organizing the microtubule cytoskeleton during interphase (PubMed:23388455). Related references: PMID:23388455

• Suppresses RAC1-mediated actin cytoskeleton reorganization and membrane protrusion formation by preventing GTP-bound RAC1 accumulation (PubMed:25074804, PubMed:28869598). Related references: PMID:25074804 PMID:28869598

• Facilitates normal cellular responses to extracellular matrix contacts and directional cell migration toward fibronectin gradients in vitro (PubMed:25074804, PubMed:28869598). Related references: PMID:25074804 PMID:28869598

• Localizes to multiple cellular compartments including nucleus, nucleolus, centromeres, mitotic spindle, midbody, and cell membrane, reflecting its diverse roles throughout the cell cycle.

• Classified as a cell cycle and mitosis regulator with acetylated and phosphorylated modifications, highlighting its dynamic regulation during cell division.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 323-522 of human RCC2, a region likely spanning functional domains critical for protein interactions; consider this when designing complementary detection reagents.
• For Western blotting, validate antibody specificity using positive (e.g., HeLa, HEK293) and negative (e.g., RCC2 knockdown) control lysates, and note the predicted molecular weight of 56 kDa.
• In ELISA applications, titrate the antibody to determine optimal working concentration and use a suitable recombinant RCC2 protein or cell lysate as coating antigen.
• Given its polyclonal nature, batch-to-batch consistency should be verified for critical quantitative assays.
• Consider cross-reactivity with RCC2 orthologs in other species if testing non-human samples, as the antibody is validated for human reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories. We offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents to support cell cycle and mitosis research, including targets like RCC2. For rare or custom raw-material needs, our integrated supply chain and technical consulting bridge the gap from concept to clinical application.