Anti-RCC1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P18754

Core Product Specifications and Parameters

Biological Background: RCC1 Function and Localization

• RCC1 acts as a guanine-nucleotide releasing factor (GEF) for the small GTPase Ran, catalyzing the exchange of GDP for GTP to regulate Ran's activity (PubMed:11336674, PubMed:17435751, PubMed:1944575).
  Related references:
  PMID:11336674, PMID:17435751, PMID:1944575

• By maintaining a high concentration of GTP-bound Ran in the nucleus, RCC1 facilitates the release of cargo proteins from importins after nuclear import (PubMed:22215983).
  Related references:
  PMID:22215983

• During mitosis, RCC1 generates chromosome-associated Ran-GTP, which is essential for proper mitotic spindle assembly and progression through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983).
  Related references:
  PMID:12194828, PMID:17435751, PMID:22215983

• RCC1 binds directly to nucleosomes and double-stranded DNA, anchoring its GEF activity to chromatin (PubMed:17435751, PubMed:18762580).
  Related references:
  PMID:17435751, PMID:18762580

• It regulates the onset of chromosome condensation during S phase, ensuring proper cell cycle progression (PubMed:3678831).
  Related references:
  PMID:3678831

• The protein contains seven tandem RCC1 repeats, forming a seven-bladed β-propeller structure, and undergoes alternative splicing to generate multiple isoforms.

• Post-translational modifications of RCC1 include phosphorylation and methylation, which may modulate its activity and interactions.

• Subcellularly, RCC1 is predominantly nuclear where it associates with chromatin, but it can also be detected in the cytoplasm.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 1-100 of human RCC1; this N-terminal region is highly conserved across human, mouse, and rat, which may account for the observed cross-reactivity.
• For Western blotting, a band corresponding to the predicted molecular weight of ~45 kDa is expected; confirm specificity using positive controls such as HeLa or HEK293 whole-cell lysates.
• In immunohistochemistry on paraffin sections, antigen retrieval is recommended, and titration may be necessary to optimize signal due to the chromatin-bound nature of the target.
• For ELISA, the same peptide immunogen can be utilized as a coating standard, or recombinant RCC1 protein may be used for quantitative assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a one-stop supply chain for IVD raw materials, including this anti-RCC1 monoclonal antibody targeting chromatin dynamics and cell cycle regulation. Our portfolio extends from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, ensuring consistent performance in your development pipeline. We also specialize in sourcing rare-target raw materials, supporting your assay design from concept to clinic.