Anti-RCC1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18754

Core Product Specifications and Parameters

Biological Background: RCC1 Function and Localization

• RCC1 (Regulator of Chromosome Condensation 1), also known as CHC1 or RCC1-I, is a guanine-nucleotide releasing factor (GEF) for the small GTPase Ran, catalyzing the exchange of GDP for GTP.
• By promoting GTP-bound Ran formation, RCC1 plays a critical role in nuclear import and mitotic spindle assembly, ensuring proper progression through mitosis. Related references: PMID:11336674 PMID:17435751 PMID:1944575
• RCC1 binds directly to nucleosomes and double-stranded DNA, which is essential for generating high local concentrations of chromosome-associated Ran-GTP during mitosis. Related references: PMID:12194828 PMID:22215983 PMID:18762580
• The protein localizes primarily to the nucleus and chromosomes, but can also be found in the cytoplasm under certain conditions.
• RCC1 undergoes multiple post-translational modifications, including phosphorylation and methylation, and alternative splicing generates different isoforms.
• UniProt keywords indicate that disease variants in RCC1 are associated with neuropathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-240) of human RCC1, which is expected to recognize the full-length protein (~45 kDa) and potentially its isoforms.
• For Western blot (WB), a band around 45 kDa is anticipated in human, mouse, and rat cell lysates; however, users should validate specificity in their experimental system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate fixation and permeabilization protocols should be optimized for the tissue or cell line of interest.
• ELISA applications may require indirect detection formats; consider pairing with a suitable secondary antibody and validate detection limits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing bridge for IVD raw materials, supporting diagnostics developers, research labs, and manufacturers from concept to clinic. This anti-RCC1 polyclonal antibody serves as a valuable tool for investigating chromosome condensation, nuclear transport, and cell cycle regulation. Beyond this antibody, CamelBio provides a comprehensive portfolio of validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing solutions for rare targets—all designed to accelerate your assay development and production timelines.