Anti-RC3H1/Roquin-1 Polyclonal Antibody for WB & ELISA - Q5TC82

Core Product Specifications and Parameters

Biological Background: Roquin-1 Function and Localization

• RC3H1 encodes Roquin-1, a member of the RING finger and CCCH-type zinc finger protein family, also known as RNF198.
• It functions as a post-transcriptional repressor by binding to constitutive decay elements (CDE) in the 3'-UTR of target mRNAs (e.g., ICOS, TNF, TNFRSF4), promoting their deadenylation and degradation. Related references: PMID:25026078, PMID:31636267
• In follicular helper T cells, Roquin-1 represses ICOS and TNFRSF4 expression, preventing spontaneous Tfh cell differentiation and autoimmunity.
• Roquin-1 also acts as a ubiquitin E3 ligase, exhibiting strongest activity with the UBE2N:UBE2V1/2 E2 complex to generate polyubiquitin chains. Related references: PMID:26489670
• It regulates microRNA homeostasis by enhancing DICER-mediated processing of pre-MIR146a and promoting mature MIR146a uridylation and turnover. Related references: PMID:25697406, PMID:31636267
• Subcellularly, Roquin-1 localizes to the cytoplasm, P-bodies, and cytoplasmic granules.
• Widely expressed across tissues, with higher levels observed in the cerebellum, spleen, ovary, and liver.
• The protein is subject to alternative splicing and phosphorylation, as indicated by UniProt annotations.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 834–1133 in the C-terminal region of human RC3H1; consider this when evaluating epitope accessibility in your assay.
• For WB, a band near 126 kDa is expected; optimize gel percentage and transfer conditions to resolve high-molecular-weight proteins.
• Use lysates from human, mouse, or rat cell lines/tissues known to express RC3H1 as positive controls.
• For ELISA, validate coating conditions and detection range using recombinant RC3H1 protein or cell lysates.
• Cross-reactivity has been reported with human, mouse, and rat; confirm reactivity in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RC3H1 polyclonal antibody supports research into post-transcriptional gene regulation and immune system function. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your development workflow.