Anti-RBPMS Rabbit Polyclonal Antibody for WB and ELISA - Q93062

Core Product Specifications and Parameters

Biological Background: RBPMS Function and Localization

• RBPMS (RNA-binding protein with multiple splicing), also known as Hermes, is an RNA-binding protein that mediates the regulation of pre-mRNA alternative splicing, acting as either an activator or repressor depending on the target transcript (PubMed:24860013, PubMed:26347403).
  Related references: PMID:24860013, PMID:26347403
• It plays a critical role in differentiated vascular smooth muscle cells by activating a splicing program that affects actin cytoskeleton and focal adhesion machineries, in cooperation with RBFOX2 and MBNL1/2.
• RBPMS binds to tandem CAC trinucleotide motifs separated by a variable spacer (1–9 nt) or to CA dinucleotide repeats, with a minimal RNA length of 15 nt, often functioning as a dimer.
• It represses TPM1 exon 3 inclusion by binding to CAC repeats and forming higher-order complexes with other splicing regulators, preventing spliceosome assembly.
• Localizes to the nucleus and cytoplasm, including stress granules and P-bodies, reflecting its dynamic role in RNA metabolism.
• Ubiquitously expressed across tissues, with highest levels in heart, prostate, small and large intestine, and ovary (PubMed:8855282).
  Related references: PMID:8855282
• Acts as a coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, enhancing SMAD2/3 phosphorylation and nuclear accumulation (PubMed:17099224).
  Related references: PMID:17099224
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.

Experimental Guidance and Technical Tips

• For Western blot (WB), use whole-cell lysates from vascular smooth muscle cells, heart, or other RBPMS-expressing tissues. The predicted molecular weight is 22 kDa; a single band near this size is typically observed, though isoform-specific patterns may appear.
• For ELISA, coat with the recombinant immunogen fragment (aa 110–219) or native protein from cell/tissue extracts. Optimize coating concentration and detection antibody dilution for best signal-to-noise.
• Given the antibody’s cross-reactivity with mouse and rat, validate in the relevant model system and consider using species-matched loading controls.
• Because RBPMS exists in multiple splice isoforms, the antibody may detect several variants; molecular weight shifts could indicate isoform-specific expression or modification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner providing diagnostic manufacturers and research teams with streamlined access to high‑quality reagents, technical support, and supply‑chain solutions—from early development to clinic. This RBPMS polyclonal antibody complements our portfolio for cardiovascular biomarker and alternative‑splicing research, and we can support your project with validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and custom sourcing for rare or emerging targets. Let CamelBio be your bridge from concept to validated assay.