Anti-RBPJK Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q06330

Core Product Specifications and Parameters

Biological Background: RBPJK Function and Localization

• Transcriptional regulator that plays a central role in Notch signaling, a pathway involved in cell-fate determinations. Acts as a repressor in the absence of Notch and as an activator when bound to the Notch intracellular domain (NICD). Related references: PMID:41086914
• Recruits chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to repress or activate transcription, respectively.
• Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
• Recognizes and binds specifically to methylated DNA. Related references: PMID:21991380
• Activates the transcription of COX4I2 under hypoxia conditions (4% oxygen) by binding to its oxygen responsive element. Related references: PMID:23303788
• Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
• Predominantly localizes to the nucleus but can also be found in the cytoplasm.
• Exists in multiple isoforms due to alternative splicing and is associated with disease variants; also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-260) of human RBPJK; consider that the antibody may recognize multiple splicing isoforms.
• For Western blot, expect a band around 56 kDa; validate in the relevant sample system and optimize loading controls.
• For IHC-P, standard antigen retrieval and appropriate blocking steps are recommended; pilot staining with known positive and negative tissues is advised.
• In ELISA, the antibody can be used for capture or detection, but optimal coating concentration and detection conditions should be determined empirically.
• Cross-reactivity with mouse and rat has been reported, but confirm expression and sequence homology in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, providing diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality reagents. This anti-RBPJK polyclonal antibody supports research in Notch signaling, transcriptional regulation, and related disease areas. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets—helping you bridge the gap from concept to clinic.