Anti-RBPJK Monoclonal Antibody for WB, IHC-P, ELISA - Q06330

Core Product Specifications and Parameters

Biological Background: RBPJK Function and Localization

• RBPJK, also known as CBF-1, RBP-J kappa, and IGKJRB1, is a key transcriptional regulator centrally involved in the Notch signaling pathway, influencing a wide array of cell-fate decisions (PubMed:41086914). Related references: PMID:41086914
• It functions as a transcriptional repressor in the absence of Notch and switches to an activator upon binding the Notch intracellular domain (NICD), modulating transcription via recruitment of histone deacetylases or acetylases.
• The protein specifically binds the immunoglobulin kappa-type J segment recombination signal sequence and can also bind to methylated DNA (PubMed:21991380). Related references: PMID:21991380
• Under hypoxic conditions (4% oxygen), RBPJK activates transcription of COX4I2 through the oxygen responsive element (PubMed:23303788). Related references: PMID:23303788
• It negatively regulates the phagocyte oxidative burst upon bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
• RBPJK localizes to both the nucleus and the cytoplasm.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human RBPJK (Q06330); verify specificity in your assay system with appropriate controls.
• For Western blot, expect a band near 56 kDa; optimize primary antibody dilution empirically for your sample type.
• In IHC-P, anticipate both nuclear and cytoplasmic staining; perform antigen retrieval and validate detection with known positive tissues.
• The antibody cross-reacts with human and mouse; if other species are used, sequence homology checks and pilot tests are recommended.
• For ELISA, the peptide immunogen can serve as a positive control; consider serial dilutions for standard curve generation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic manufacturers and labs from concept to clinic. This anti-RBPJK rabbit monoclonal antibody is a valuable tool for Notch signaling and transcriptional regulation research. Beyond antibodies, we supply validated antibody pairs, bulk reagents, and customized sourcing for rare targets, helping you accelerate assay development with reliable raw material solutions.