Anti-RBP4 Rabbit Polyclonal Antibody for WB, ELISA - P02753

Core Product Specifications and Parameters

Biological Background: RBP4 Function and Localization

• Retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein (PRBP or RBP), is a secreted carrier of the lipocalin family that specifically transports retinol (vitamin A) in blood plasma. Related references: PMID:5541771
• Once loaded with retinol in the liver, RBP4 circulates and delivers the vitamin to peripheral tissues, playing an essential role in vision, cellular differentiation, and vitamin A homeostasis.
• RBP4 transfers its bound all-trans retinol to the STRA6 receptor on target cells, which then facilitates retinol transport across the cell membrane. Related references: PMID:22665496
• The protein is secreted and detected in blood plasma and urine, consistent with its transport function and renal clearance.
• RBP4 contains a single disulfide bond and undergoes post-translational methylation; mutations in RBP4 are associated with microphthalmia, underscoring its critical role in ocular development.
• The mature protein has a calculated molecular weight of approximately 23 kDa, and typically migrates around 21 kDa under reducing SDS-PAGE conditions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 19–201 of human RBP4, corresponding to the mature secreted form, so the antibody is expected to recognize both full-length and processed protein.
• For Western blot, consider using human plasma, serum, or liver lysates as positive controls; a band near 21 kDa may be observed. Optimize primary antibody dilution and detection conditions empirically.
• For ELISA, direct or sandwich formats may be tested; titrate the antibody against recombinant RBP4 or reference serum samples to determine optimal working concentrations.
• The antibody shows cross-reactivity with mouse RBP4, making it suitable for studies in mouse models, but validation in the relevant sample type is recommended.
• To maintain stability, aliquot and store at -20°C or -80°C, avoiding repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions streamlined access to a broad portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For researchers investigating retinol transport, vitamin A metabolism, or vision-related disorders, CamelBio can source polyclonal and monoclonal antibodies, matched antibody pairs, and bulk quantities to support assay development. With a focus on supply continuity and technical support, CamelBio bridges the gap from concept to clinic.