Anti-RBP3 Rabbit Polyclonal Antibody for WB and ELISA - P10745

Core Product Specifications and Parameters

Biological Background: RBP3 Function and Localization

• Retinol-binding protein 3 (RBP3), also known as interphotoreceptor retinoid-binding protein (IRBP), is a secreted glycoprotein that plays a critical role in the visual cycle by transporting 11-cis- and all-trans-retinoids between the retinal pigment epithelium and photoreceptors.
• RBP3 is a member of the retinol-binding protein family and is encoded by the RBP3 gene on human chromosome 10 (gene ID 5949).
• The protein is secreted into the interphotoreceptor matrix, the extracellular space between the neural retina and the retinal pigment epithelium, where it facilitates retinoid exchange essential for phototransduction.
• RBP3 contains internal repeats that likely contribute to its retinoid-binding capacity and is post-translationally modified by glycosylation.
• Mutations in RBP3 are associated with retinitis pigmentosa, a group of inherited retinal degenerative diseases, highlighting its importance in retinal health.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers amino acids 18–320 of human RBP3, which includes the N-terminal region and likely contains important functional domains; this design may contribute to specific recognition.
• For Western blot (WB), the predicted molecular weight of RBP3 is approximately 135 kDa; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In ELISA applications, the antibody may be useful for detecting recombinant or native RBP3; users should consider validating the assay with relevant controls and standards.
• Cross-reactivity with mouse and rat RBP3 has been confirmed by the supplier, making this antibody suitable for studies in rodent models of retinal function.
• For immunodetection, use sample preparation methods that preserve the extracellular matrix localization of RBP3 (e.g., native conditions or gentle fixation) to maintain antigen integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop shop for IVD raw materials, supporting diagnostic manufacturers and research labs with validated antibody pairs, bulk ancillary reagents, and specialized sourcing of rare targets like RBP3 for visual cycle research. Our portfolio includes this rabbit polyclonal antibody, which is suitable for immunoassay development, and we can assist with customized sourcing needs to accelerate your diagnostic workflows. Partner with CamelBio to streamline your procurement from concept to clinic.