Anti-RBP1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P09455

Core Product Specifications and Parameters

Biological Background: RBP1 Function and Localization

• RBP1 encodes cellular retinol-binding protein I (CRBP-I), a cytoplasmic carrier that accepts retinol from STRA6 and contributes to retinol uptake, storage, and retinoid homeostasis. Related references: PMID:22665496 PMID:26900151 PMID:28057518
• Following uptake, CRBP-I binds retinol delivered by the membrane receptor STRA6, facilitating intracellular transport and metabolic channeling. Related references: PMID:15632377 PMID:22665496
• The protein localizes predominantly to the cytoplasm and is also found associated with lipid droplets, reflecting its role in retinoid trafficking.
• Tissue expression is widespread, with notably higher levels in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.
• Alternative splicing generates multiple RBP1 isoforms; the protein belongs to the intracellular lipid-binding protein family.
• Post-translational methylation has been identified, though its functional significance remains under investigation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RBP1 (aa 1–135), making this polyclonal antibody likely to recognize multiple epitopes; it is suitable for both denaturing (WB) and native (IF/ICC, IF-P, ELISA) applications.
• For Western blotting, the predicted molecular weight is ~16 kDa; consider using tissue lysates with known high expression (e.g., liver, ovary) as positive controls.
• In immunofluorescence experiments, ensure adequate permeabilization to detect cytoplasmic and lipid-droplet-associated RBP1; co-staining with organelle markers may confirm localization.
• Given reported cross-reactivity with mouse and rat, validate the antibody in your specific experimental system with appropriate species-matched controls.
• Optimization of antibody dilution and incubation conditions is recommended for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a dedicated sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies (such as this RBP1 rabbit polyclonal antibody), bulk ancillary reagents, and targeted procurement for rare biomarkers in retinoid metabolism and vitamin A transport research. Contact us to streamline your IVD development with reliable, quality-assured reagent supply.