Anti-RBMY1F Polyclonal Antibody for WB and ELISA - Q15415

Core Product Specifications and Parameters

Biological Background: RBMY1F Function and Localization

• RBMY1F, also known as Y chromosome RNA recognition motif 2 (YRRM2), is an RNA-binding protein encoded by the RBMY1F gene (and alternatively RBMY1J) located on the Y chromosome.
• It belongs to the RBMY family of RNA-binding motif proteins, which are specifically expressed in the testis and essential for male germ cell development.
• RBMY1F is primarily involved in spermatogenesis, functioning as a potential pre-mRNA splicing factor in the testis to regulate gene expression during sperm development.
• The protein localizes exclusively to the nucleus, consistent with its role in RNA processing and splicing events.
• According to UniProt keywords, RBMY1F participates in alternative splicing and mRNA processing, indicating its regulatory role in transcript diversity within testicular cells.
• Tissue specificity analysis reveals that RBMY1F expression is restricted to testis, underscoring its specialized function in reproductive biology.
• The protein has a calculated molecular weight of ~55.7 kDa (UniProt), while the immunogen-derived product detects a band at 56kDa, consistent with the full-length form.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering the N-terminal 1-80 amino acids of human RBMY1F. This region may be critical for epitope recognition; consider sequence alignment if testing other species.
• For Western blot, a band of approximately 56kDa is expected in human testis lysates; rat samples may also show reactivity due to cross-reactivity.
• For ELISA, use recombinant RBMY1F protein or peptide as coating antigen to assess antibody titer and specificity.
• As the target is testis-specific, validate the antibody in appropriate human or rat testis tissue/cell extracts; non-testis samples should show no or low signal.
• Pre-adsorption with the immunogen can be used as a negative control in immunodetection assays to confirm specificity.
• Due to polyclonal nature, the antibody may recognize multiple epitopes; consider affinity purification if needed for specific applications.
• Note that no specific dilutions or quantitative validation is provided; optimization in the end-user's experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw-material solutions, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-RBMY1F polyclonal antibody is ideal for reproductive biology and spermatogenesis research, and CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. We also excel in sourcing rare target reagents to meet your unique project needs.