Anti-RBM7 Polyclonal Antibody for WB, ELISA - Q9Y580

Core Product Specifications and Parameters

Biological Background: RBM7 Function and Localization

• RBM7 (RNA-binding motif protein 7) is an RNA-binding protein that serves as the RNA-binding subunit of the nuclear exosome targeting (NEXT) complex, which directs non-coding short-lived RNAs for exosomal degradation (PubMed:25189701, PubMed:25525152, PubMed:25578728). Related references: PMID:25189701, PMID:25525152, PMID:25578728
• The NEXT complex is involved in surveillance and turnover of aberrant transcripts and non-coding RNAs, including promoter upstream transcripts (PROMPTs), enhancer RNAs (eRNAs), and 3'-extended small nuclear RNAs (snRNAs) (PubMed:25189701, PubMed:25852104, PubMed:27871484). Related references: PMID:25189701, PMID:25852104, PMID:27871484
• RBM7 preferentially binds polyuridine sequences and associates with newly synthesized RNAs, facilitating substrate recognition for the NEXT complex (PubMed:25189701, PubMed:25525152, PubMed:25578728). Related references: PMID:25189701, PMID:25525152, PMID:25578728
• During DNA damage response (DDR), RBM7 interacts with MEPCE and LARP7, core subunits of 7SK snRNP, leading to release of P-TEFb complex and induction of DDR gene transcription (PubMed:30824372). Related references: PMID:30824372
• Under stress conditions, activation of the p38MAPK-MK2 pathway decreases RBM7 RNA-binding activity, thereby modulating the turnover of the non-coding transcriptome (PubMed:25525152). Related references: PMID:25525152
• RBM7 localizes to the nucleoplasm and nucleus, consistent with its role in nuclear RNA processing and degradation.
• Keywords from UniProt include acetylation, methylation, phosphorylation, and meiosis, indicating post-translational modifications that may regulate its function.
• Ubiquitously expressed, suggesting a general role in RNA metabolism across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RBM7 protein (amino acids 1–266). The resulting polyclonal antibody is expected to recognize multiple epitopes, providing robust detection in applications such as Western blot and ELISA.
• For Western blot, consider using whole-cell lysates from human or mouse samples; RBM7 migrates at ~31 kDa. Validate in the relevant sample system and optimize antibody dilution.
• In ELISA, this antibody can be used for direct detection or as a capture reagent for recombinant RBM7 protein. Due to its polyclonal nature, batch-to-batch variability may occur; it is advisable to titrate and validate the antibody for each new lot in your specific assay.
• Cross-reactivity with mouse has been confirmed, enabling studies in murine models. No reactivity data with other species are available; verify if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Our RBM7 polyclonal antibody supports research in RNA biology and next-generation diagnostic development, backed by consistent quality and supply chain reliability. Whether you need customized antibody panels or technical consulting from concept to clinic, CamelBio bridges your sourcing needs with expert support.