Anti-Rbm7 (KO Validated) Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q9CQT2

Core Product Specifications and Parameters

Biological Background: Rbm7 Function and Localization

• Rbm7 (RNA-binding protein 7) is a ~30 kDa RNA recognition motif-containing protein that functions as the RNA-binding subunit of the trimeric nuclear exosome targeting (NEXT) complex.
• The NEXT complex directs a subset of non-coding short-lived RNAs for exosomal degradation, playing a key role in surveillance and turnover of aberrant transcripts and non-coding RNAs.
• Rbm7 binds preferentially to polyuridine sequences and associates with newly synthesized RNAs, including pre-mRNAs, promoter upstream transcripts (PROMPTs), enhancer RNAs (eRNAs), and 3'-extended products from small nuclear RNAs (snRNAs).
• It participates in DNA damage response (DDR) by interacting with MEPCE and LARP7, core subunits of the 7SK snRNP complex, leading to the release of positive transcription elongation factor b (P-TEFb) and transcription of DDR genes, thereby promoting cell viability.
• During stress response, activation of the p38MAPK-MK2 pathway decreases Rbm7 RNA-binding and subsequent RNA exosome degradation activities, modulating non-coding transcriptome turnover.
• Subcellular localization is predominantly nuclear, with presence in the nucleoplasm.
• Post-translational modifications include acetylation, methylation, and phosphorylation; keywords also highlight roles in meiosis.

Experimental Guidance and Technical Tips

• This antibody has been validated in knockout (KO) mouse models, making it suitable for confirming target specificity in knockout vs. wild-type samples; include appropriate positive and negative controls.
• For Western blot (WB), a starting dilution of 1:1000 is commonly used; optimize for your sample type. The predicted molecular weight is 30 kDa; verify band size with a molecular weight marker.
• The immunogen sequence (aa 87-265) is conserved between mouse and rat, consistent with observed cross-reactivity; cross-reactivity with other species has not been tested and requires individual validation.
• For immunoprecipitation (IP), use a protein A/G bead system and pre-clear lysates; note that RNA-binding proteins may require gentle lysis conditions to preserve interactions.
• For immunostaining (IF-P, IHC-P), antigen retrieval may be necessary; perform pilot experiments on known positive tissues to optimize conditions.
• In ELISA, consider using recombinant Rbm7 protein as a coating antigen to establish a standard curve; validate linearity and detection limit in your assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials. For researchers focusing on RNA processing, transcriptome surveillance, or DNA damage response pathways, this anti-Rbm7 rabbit monoclonal antibody provides a reliable tool for studying nuclear exosome targeting mechanisms. Our supply capabilities are designed to bridge the gap from basic research to diagnostic assay development, ensuring consistent quality and scalable availability.