Anti-RBM6 Rabbit Polyclonal Antibody for WB and ELISA - P78332

Core Product Specifications and Parameters

Biological Background: RBM6 Function and Localization

• RBM6 (RNA-binding protein 6) is a member of the RNA-binding motif (RBM) protein family, specifically binding poly(G) RNA homopolymers in vitro.
• Also known as lung cancer antigen NY-LU-12 and protein G16, it is recognized as a tumor-associated antigen with relevance in lung cancer research.
• The protein predominantly localizes to the nucleus, consistent with its role in RNA metabolism and gene regulation.
• RBM6 is ubiquitously expressed in adult tissues, indicating a fundamental cellular function.
• Post-translational modifications include phosphorylation and ubiquitin conjugation (isopeptide bond formation), which may regulate its activity or stability.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 530–770 (NP_005768.1), which may include conserved functional domains; validate specificity with appropriate positive and negative controls.
• For Western blot, expect a band near 129 kDa; consider using human cell lysates known to express RBM6 and verify with siRNA knockdown or overexpression if needed.
• In ELISA applications, use recombinant RBM6 protein or peptide fragments corresponding to the immunogen as a coating antigen to optimize sensitivity.
• As a polyclonal antibody, it may recognize multiple isoforms due to alternative splicing; confirm band patterns in relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-RBM6 rabbit polyclonal antibody supports oncology and RNA biology research, and we can source tailored reagent packages to accelerate your assay development from concept to clinic.