Anti-RBM5 Polyclonal Antibody for WB, ELISA - P52756

Core Product Specifications and Parameters

Biological Background: RBM5 Function and Localization

• RBM5 (RNA-binding protein 5), also known as G15 and LUCA15, is a putative tumor suppressor and component of the spliceosome A complex.
• It binds single-stranded RNA with the consensus sequence 5'-AGGUAA-3'. Related references: PMID:21256132
• RBM5 regulates alternative splicing of pre-mRNAs by modulating splice site pairing after recruitment of U1 and U2 snRNPs.
• The protein plays a dual role in apoptosis: promotes exclusion of exon 6 in FAS to produce soluble FAS (inhibiting apoptosis), and exclusion of exon 9 in CASP2 to produce active caspase-2 (promoting apoptosis).
• RBM5 localizes predominantly to the nucleus, consistent with its function in pre-mRNA splicing.
• Isoform 5 is widely expressed in normal tissues and shows elevated levels in T-leukemic cell lines.
• The protein contains zinc-finger domains, indicating metal-binding capability, and is subject to phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–130) of human RBM5; consider validating specificity in relevant sample systems.
• For western blotting, the predicted molecular weight is 92 kDa; nuclear extracts may be advantageous due to the protein's localization.
• This antibody cross-reacts with mouse and rat RBM5, making it suitable for cross-species studies in appropriate tissue or cell models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials like this anti-RBM5 polyclonal antibody, covering targets relevant to apoptosis and splicing. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for large-scale assay development. Trust CamelBio for reliable sourcing of rare-target raw materials, empowering your transition from concept to clinic.