Anti-RBM3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P98179

Core Product Specifications and Parameters

Biological Background: RBM3 Function and Localization

• RBM3 is an RNA-binding protein also known as RNA-binding motif protein 3 (RNPL), acting as a cold-inducible factor that enhances global protein synthesis under both physiological and mild hypothermic conditions.
• It modulates microRNA abundance (when overexpressed) and enhances phosphorylation of translation initiation factors and polysome formation, collectively supporting cap-dependent translation.
• The protein localizes to the nucleus, cytoplasm, and dendrites (cell projections), indicating roles in both transcriptional and translational regulation.
• Post-translational modifications include phosphorylation and methylation, as identified by direct protein sequencing and proteomics.
• RBM3 is classified as a stress response protein, induced by cold stress, and is part of the RNA-binding reference proteome.
• Three-dimensional structural studies have been performed (keyword: 3D-structure).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the N-terminal region (aa 1-157) of human RBM3. When using this antibody, consider that it may recognize epitopes within this region.
• For Western blot (WB), a band at approximately 17 kDa is expected for RBM3 in human, mouse, and rat samples.
• The antibody is applicable to immunohistochemistry (paraffin-embedded sections) and ELISA; validate in the relevant sample system to confirm staining specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For stress response and RNA-binding protein research, CamelBio offers reliable sourcing solutions for RBM3 and related targets, supporting your transition from concept to clinic.