Anti-RBM25 Polyclonal Antibody for WB, IF/ICC, ELISA - P49756

Core Product Specifications and Parameters

Biological Background: RBM25 Function and Localization

• RBM25 is an RNA-binding protein with aliases including RED120, S164, and RNPC7, encoded by the RBM25 gene (GeneID: 58517).
• It acts as a key regulator of alternative pre-mRNA splicing, directly binding to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence.
• RBM25 critically modulates apoptotic cell death by controlling the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L.
• It promotes the usage of the BCL2L1-S 5'-splice site through cooperation with LUC7L3, facilitating U1 snRNP recruitment to weak splice sites.
• In heart failure, RBM25 is implicated in generating an abnormal truncated splice variant of SCN5A, linking it to cardiac pathophysiology.
• The protein localizes to nuclear speckles and the cytoplasm, consistent with its roles in mRNA processing and splicing regulation.
• RBM25 undergoes multiple post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 614-843 of human RBM25; verify sequence conservation in mouse if cross-reactivity is critical for your experiment.
• For Western blotting, expect a band around 100kDa and optimize blocking and antibody dilution using a suitable positive control lysate.
• In immunofluorescence, nuclear speckle staining is anticipated; include negative controls to confirm specificity and minimize background.
• ELISA-based assays may require titration of both capture/detection antibodies and antigen; validate using recombinant protein or cell extracts expressing RBM25.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies targeting key regulators of RNA processing and apoptosis. Our one-stop sourcing model supports your assay development with optimized polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents, ensuring reliable supply from concept to clinic. Whether you need routine or rare targets such as RBM25, CamelBio streamlines procurement to accelerate your diagnostic and research projects.