Anti-RBM22 Rabbit Polyclonal Antibody for WB and ELISA - Q9NW64

Core Product Specifications and Parameters

Biological Background: RBM22 Function and Localization

• RBM22 (RNA-binding motif protein 22, also known as Cwc2, ZC3H16, fSAP47) is a pre-mRNA-splicing factor required for the first step of splicing as a component of the activated spliceosome. Related references: PMID:28076346, PMID:28502770, PMID:29301961
• It binds directly to the internal stem-loop (ISL) domain of U6 snRNA and to the pre-mRNA intron near the 5' splice site during activation and catalysis.
• RBM22 contributes to the cellular stress response by mediating the translocation of SLU7 to the cytoplasm and PDCD6 to the nucleus.
• Subcellularly, RBM22 localizes to both the nucleus and cytoplasm, consistent with its roles in splicing and transport.
• The protein features a CCCH-type zinc finger domain, conferring RNA-binding capability and classifying it as a zinc finger protein.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may regulate its function in spliceosome dynamics.

Experimental Guidance and Technical Tips

• The immunogen spans full-length human RBM22 (aa 1–230), enabling detection of endogenous RBM22 in species with high sequence conservation (human, mouse, rat cross-reactivity confirmed).
• In Western blot (WB), the predicted molecular weight is 47 kDa; verify specificity with appropriate controls and consider using nuclear or total cell lysates.
• For ELISA, optimization of coating antigen and antibody dilution is recommended; the recombinant immunogen can serve as a positive control.
• Given its role in splicing and stress, nuclear/cytoplasmic fractionation or stress induction may enhance functional insights, but validation in your specific model is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a streamlined supply chain for IVD raw materials, from discovery to clinical application. This RBM22 polyclonal antibody exemplifies our commitment to providing high-quality reagents for splicing and RNA processing research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.