Anti-RBM19 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y4C8

Core Product Specifications and Parameters

Biological Background: Probable RNA-binding protein 19 Function and Localization

• RBM19, also known as RNA-binding motif protein 19, is a probable RNA-binding protein implicated in developmental processes.
• It plays a critical role in embryo pre-implantation development, as suggested by functional studies.
• Subcellularly, RBM19 localizes to the nucleus, specifically the nucleolus and nucleoplasm, with additional presence in the cytoplasm and association with chromosomes.
• It is classified as a developmental protein and phosphoprotein, indicating potential regulation by phosphorylation.
• RBM19 contains RNA-binding motifs and is subject to ubiquitin-like conjugation (isopeptide bond), suggesting involvement in RNA metabolism and post-translational control.
• Tissue expression studies show RBM19 is present in the crypts of Lieberkühn of the intestine and in intestinal neoplasia, hinting at a role in intestinal epithelial biology and cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 759-960) near the C-terminus of human RBM19, which may help target full-length protein detection without N-terminal isoform interference.
• For Western blotting (WB), a band at approximately 107 kDa is expected; include positive controls such as intestinal epithelial cell lysates where RBM19 is expressed.
• In ELISA applications, consider evaluating the antibody as both capture and detection reagent; proper titration and validation with recombinant RBM19 protein are recommended.
• Because RBM19 is a nuclear and cytoplasmic protein, subcellular fractionation may enhance detection in WB when using limited samples.
• Cross-reactivity with other species has not been tested; for non-human studies, verify sequence homology of the immunogen region before use.

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CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our catalog includes validated antibodies against critical developmental and cancer targets like RBM19, paired with reliable sourcing for ancillary reagents and rare-target raw materials. Whether you need bulk monoclonal antibodies, custom recombinant proteins, or integrated assay development support, CamelBio simplifies your supply chain to accelerate diagnostic innovation.