Anti-RBM17 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96I25

Core Product Specifications and Parameters

Biological Background: RBM17 Function and Localization

• Splicing factor 45 (RBM17), also known as RNA-binding motif protein 17, is a ~45 kDa protein that functions in pre-mRNA splicing as part of the spliceosome.
• Binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step of splicing.
• Plays a role in alternative splicing regulation and can promote the use of cryptic splice sites. For instance, it facilitates a cryptic splice site generated by the beta-110 mutation in the HBB gene, which leads to sickle cell anemia.
• Localizes predominantly to the nucleus, consistent with its role in mRNA processing.
• Subject to post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation).
• Identified as a proteomics target and reference proteome member with RNA-binding activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-240 of human RBM17 (N-terminal region), which should recognize both full-length and truncated forms of the protein.
• For Western blot, expect a band around 45 kDa in human, mouse, and rat cell or tissue lysates. Consider using appropriate loading controls.
• The antibody is applicable for immunohistochemistry (paraffin-embedded sections) and ELISA. Optimize concentrations and detection systems for your specific sample type.
• Due to its nuclear localization, ensure proper nuclear extraction or antigen retrieval methods for IHC-P.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The RBM17 polyclonal antibody supports research on splicing mechanisms and related pathologies. For rare targets or customized sourcing needs, CamelBio facilitates a streamlined procurement process from concept to clinic.