Anti-RBM15B Rabbit Polyclonal Antibody for WB and ELISA - Q8NDT2

Core Product Specifications and Parameters

Biological Background: RBM15B (Putative RNA-binding protein 15B) Function and Localization

• Acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs by serving as an associated component of the WMM complex that mediates m6A methylation, influencing RNA splicing and processing.
  Related references:
  PMID:27602518
• Plays a crucial role in X chromosome inactivation by binding Xist RNA and recruiting the WMM complex for m6A methylation, which promotes transcriptional repression of Xist.
  Related references:
  PMID:27602518
• Regulates alternative or illicit splicing, potentially through its role in m6A methylation.
  Related references:
  PMID:16129689
• Inhibits pre-mRNA splicing.
  Related references:
  PMID:21044963
• Serves as a cofactor for the nuclear export receptor NXF1, facilitating mRNA export from the nucleus.
  Related references:
  PMID:19586903
• Localizes to the nucleoplasm, nuclear speckles, and nuclear envelope.
• Ubiquitously expressed across tissues.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (UniProt Keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 540–720 of human RBM15B (NP_037418.3). Consider using this recombinant fragment as a positive control in ELISA or other immunoassays.
• For Western blotting, the predicted molecular weight is approximately 97 kDa. Validate the antibody in your sample system, as endogenous expression levels and post-translational modifications may affect band migration.
• Given the cross-reactivity with human and mouse, this antibody is suitable for studies in mouse models, but species-specific validation is recommended.
• The antibody is polyclonal, so lot-to-lot consistency should be monitored; consider using recombinant monoclonal alternatives for assays requiring high reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to specialized IVD raw materials like the RBM15B Rabbit pAb, supporting studies in RNA epigenetics and m6A methylation. Our one-stop platform offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for large-scale assay development. For rare targets or customized sourcing, our supply network helps bridge research concepts to clinical applications.