Anti-RBM15 Polyclonal Antibody for WB, IHC-P, ELISA - Q96T37

Core Product Specifications and Parameters

Biological Background: RBM15 Function and Localization

• RBM15, also known as OTT1 and SPEN, is an RNA-binding protein and an associated component of the WMM complex, which mediates N6-methyladenosine (m6A) methylation of RNAs. Related references: PMID:27602518
• It acts as a key regulator of m6A methylation, possibly by binding target RNAs and recruiting the WMM complex, thereby influencing mRNA splicing and RNA processing. Related references: PMID:27602518
• RBM15 is essential for random X chromosome inactivation by binding Xist RNA, mediating m6A methylation, and recruiting the YTHDC1 reader to promote transcriptional repression. Related references: PMID:27602518
• It regulates alternative splicing of genes critical for megakaryocyte differentiation, likely through m6A modification. Related references: PMID:26575292
• RBM15 functions as an mRNA export factor, facilitating the export and expression of RTE-containing mRNAs prior to splicing, and may be implicated in HOX gene regulation. Related references: PMID:17001072 PMID:19786495 PMID:11344311
• Subcellularly, RBM15 localizes to nuclear speckles, nucleoplasm, nuclear envelope, and nuclear membrane, consistent with its roles in RNA metabolism and transport.
• It is required for the development of multiple tissues, including placental vascular branching morphogenesis and embryonic heart and spleen.
• RBM15 is subject to multiple post-translational modifications including acetylation, methylation, and phosphorylation, and is annotated as a proto-oncogene; it is also implicated in chromosomal rearrangements and host-virus interactions.

Experimental Guidance and Technical Tips

• For Western blotting, consider the predicted molecular weight of 107 kDa; validate band specificity using relevant cell lines or tissues expressing RBM15.
• When performing immunohistochemistry on paraffin-embedded sections, optimized antigen retrieval methods (e.g., heat-induced epitope retrieval) may enhance detection; pilot experiments with known positive controls are recommended.
• The immunogen spans amino acids 530-780 in the C-terminal region; this antibody is expected to detect full-length RBM15 and potentially isoforms containing this epitope.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; lot-specific validation in the intended application is advised.
• For ELISA, pair with a monoclonal capture antibody if developing a sandwich assay; titrate both antibodies for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This RBM15 antibody supports research into m6A methylation, X chromosome inactivation, and hematopoietic differentiation; CamelBio can also assist in sourcing rare-target reagents for your developmental and epigenetic studies. Partner with us to streamline your supply chain from concept to clinic.