Anti-RBM14 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96PK6

Core Product Specifications and Parameters

Biological Background: RBM14 Function and Localization

• RBM14 (RNA-binding protein 14), also known as COAA, PSP2, and SYTIP1, is a nuclear RNA-binding protein with two major isoforms arising from alternative splicing.
• Isoform 1 functions as a transcriptional coactivator via NCOA6 and CITED1, while isoform 2 acts as a repressor, modulating transcription. Related references: PMID:11443112
• Plays a critical role in centriole biogenesis by suppressing aberrant STIL-CPAP complexes, thus maintaining mitotic spindle integrity. Related references: PMID:25385835
• Participates in the innate immune response to DNA viruses by forming the HDP-RNP complex, which facilitates IRF3 phosphorylation and cGAS-STING signaling. Related references: PMID:28712728
• Contributes to pre-mRNA alternative splicing regulation. Related references: PMID:37548402
• Primarily localizes to the nucleus and nucleolus but can be found in the cytoplasm.
• Ubiquitously expressed in human tissues including brain, heart, kidney, spleen, and lymphocytes.
• Post-translationally modified by phosphorylation and acetylation; contains UG repeats and RRM domains characteristic of RNA-binding proteins.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein spanning amino acids 110–220 of human RBM14. It may recognize both isoforms in human, mouse, and rat samples, but cross-reactivity with other species should be independently confirmed.
• For Western blot, a band near 69 kDa is expected in lysates from cell lines or tissues expressing RBM14. Optimize primary antibody dilution (e.g., 1:500–1:2000) and use appropriate blocking conditions; validate with positive and negative controls.
• In immunofluorescence/ICC, expect nuclear and nucleolar staining, potentially with weak cytoplasmic signal. Include nuclear counterstains and test permeabilization conditions.
• For ELISA applications, the antibody can be used as a detection or capture reagent; titrate to determine optimal working concentration. Consider pairing with a different clone for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to a wide range of IVD raw materials, including validated polyclonal antibodies like this anti-RBM14 product. Supporting studies in transcriptional regulation, cell cycle, and innate immunity, CamelBio streamlines your sourcing with consistent supply chains, from single reagents to bulk orders, alongside expert technical consultation.