Anti-RBM14 Polyclonal Antibody for WB, ELISA - Q96PK6

Core Product Specifications and Parameters

Biological Background: RBM14 Function and Localization

• Isoform 1 acts as a nuclear receptor coactivator, enhancing transcription via NCOA6 and CITED1, while isoform 2 functions as a transcriptional repressor modulating coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Related references: PMID:11443112
• Regulates centriole biogenesis by suppressing formation of aberrant centriolar protein complexes in the cytoplasm, preserving mitotic spindle integrity; prevents STIL-CPAP complex formation by interfering with STIL-CPAP interaction (PubMed:25385835). Related references: PMID:25385835
• Involved in DNA virus-mediated innate immune response by assembling into HDP-RNP complex, which facilitates IRF3 phosphorylation and activates innate immunity via cGAS-STING pathway (PubMed:28712728). Related references: PMID:28712728
• Plays a role in regulation of pre-mRNA alternative splicing (PubMed:37548402). Related references: PMID:37548402
• Alternative names include Paraspeckle protein 2 (PSP2), RNA-binding motif protein 14, RRM-containing coactivator activator/modulator, and Synaptotagmin-interacting protein (SYT-interacting protein), reflecting its diverse interactions.
• Located in the nucleus, nucleolus, and cytoplasm, indicating shuttling between compartments.
• Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, and peripheral blood lymphocytes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond), as suggested by keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 110-220) of human RBM14. For WB, consider using lysates from cell lines expressing RBM14 (e.g., HeLa, 293T) as positive controls.
• Because the antibody is polyclonal, it may recognize multiple epitopes; validate specificity by knockdown or overexpression experiments.
• Predicted molecular weight is 69 kDa; in WB, a band near 69 kDa is expected, but isoform variations or post-translational modifications may cause slight shifts.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers comprehensive support from concept to clinic. For RBM14 and related transcription/immune targets, we supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Leverage our one-stop sourcing bridge to accelerate your diagnostic development with confidence.