Anti-RBM11 Rabbit Polyclonal Antibody for WB and ELISA - P57052

Core Product Specifications and Parameters

Biological Background: RBM11 Function and Localization

• Splicing regulator RBM11, also known as RNA-binding motif protein 11, is a tissue-specific splicing factor involved in the regulation of alternative splicing during neuron and germ cell differentiation.
• RBM11 antagonizes SRSF1-mediated BCL-X splicing, suggesting a role in modulating apoptotic pathways through alternative splicing control.
• It may influence the selection of alternative 5' splice sites by binding to specific exonic sequences and counteracting the SR protein SRSF1.
• The protein localizes to the nucleus, specifically to the nucleoplasm and nuclear speckles, consistent with its function in pre-mRNA processing.
• RBM11 is predominantly expressed in brain regions (including hippocampus, prefrontal cortex, and cerebellum), spinal cord, and testis, with additional expression in mammary gland, spleen, and kidney; fetal brain also shows expression.
• As a developmental protein with RNA-binding activity, it likely contributes to the fine-tuning of gene expression programs during cellular differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RBM11 sequence (aa 1-281), suggesting the antibody may recognize multiple epitopes; consider using appropriate blocking and washing conditions for WB.
• Due to endogenous expression in brain and testis, tissue lysates from these organs (human, mouse, or rat) can serve as positive controls; validate specificity using knock-down or knock-out models where possible.
• For ELISA applications, ensure optimal coating antigen concentration and antibody dilution by preliminary titration; cross-reactivity with mouse and rat may facilitate comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials like this RBM11 antibody, supporting studies in alternative splicing and neurodevelopment. Our portfolio includes validated antibody pairs, bulk reagents, and rare-target sourcing capabilities to accelerate your assay development. Partner with CamelBio to bridge the gap from concept to clinic with reliable, scalable supply solutions.