Anti-RBL2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q08999

Core Product Specifications and Parameters

Biological Background: RBL2 Function and Localization

• RBL2, also known as retinoblastoma-like protein 2, p130, or RBR-2, is a 128 kDa member of the retinoblastoma protein family.
• It functions as a key regulator of cell cycle entry, acting as a potent inhibitor of E2F-mediated transcription, preferentially binding to E2F5.
• RBL2 is directly involved in heterochromatin formation by maintaining chromatin structure and stabilizing histone methylation, particularly controlling histone H4 lysine 20 trimethylation through recruitment of methyltransferases KMT5B and KMT5C.
• It binds to cyclins A and E, and may interact with adenovirus E1A protein, potentially influencing host-virus interactions.
• Subcellularly, RBL2 localizes to the nucleus, consistent with its role in transcriptional repression and chromatin regulation.
• The protein functions as a tumor suppressor, and mutations are associated with disease variants and intellectual disability (UniProt keywords).
• Post-translational modifications include phosphorylation (phosphoprotein), and it is classified as a glycoprotein and DNA-binding protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 413–613 of human RBL2, which may provide specificity for this region. Consider verifying reactivity in your target species and system.
• For Western blot (WB), the predicted molecular weight is 128 kDa. Optimize blocking conditions and primary antibody dilution for your sample type.
• In IF/ICC experiments, employ appropriate nuclear permeabilization protocols and include positive/negative controls to confirm staining specificity.
• Cross-reactivity is reported with human and mouse; if using other species, validate by sequence alignment and experimental testing.

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CamelBio connects diagnostic manufacturers and research labs with high-quality IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-RBL2, and bulk ancillary reagents. For cell cycle and oncology projects, we can source rare-target antibodies, recombinant proteins, and custom immunoreagents to accelerate your concept-to-clinic development. Contact us to discuss your specific procurement needs.