Anti-RBFOX1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9NWB1

Core Product Specifications and Parameters

Biological Background: RBFOX1 Function and Localization

• RBFOX1 (RNA binding protein fox-1 homolog 1), also designated Ataxin-2-binding protein 1 (A2BP1), Fox-1 homolog A, or Hexaribonucleotide-binding protein 1, is an RNA-binding protein encoded by the RBFOX1 gene.
• It functions as a regulator of alternative splicing by recognizing and binding to the conserved 5'-UGCAUGU-3' motif within pre-mRNA molecules.
• RBFOX1 is involved in the tissue-specific regulation of exon inclusion and plays a role in the splicing of differentially expressed exons during erythroid differentiation.
• Predominantly expressed in muscle and brain, the protein is critical for the specialized splicing programs in these tissues.
• Subcellularly, RBFOX1 is found in both the nucleus and cytoplasm, reflecting its roles in RNA processing and potential cytoplasmic functions.
• UniProt keywords include alternative splicing, mRNA processing, mRNA splicing, RNA-binding, methylation, and reference proteome, highlighting its involvement in post-transcriptional regulation.
• The protein has a calculated molecular weight of approximately 42.8 kDa and an observed band around 43 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 130 amino acids (NP_665900.1); consider this region when designing competition or domain-specific studies.
• This polyclonal antibody is suitable for Western blotting, immunofluorescence on paraffin sections (IF-P), and ELISA; optimal working dilutions should be determined empirically.
• Cross-reactivity has been observed with human, mouse, and rat samples; for other species, analyze sequence identity of the immunogen region before use.
• As with all polyclonal antibodies, lot-to-lot consistency should be verified for critical applications; consider including positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined sourcing channel for IVD raw materials. This anti-RBFOX1 polyclonal antibody supports splicing and neurobiology research, and we can supply validated antibody pairs, optimized monoclonal alternatives, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, leverage our integrated procurement to accelerate your assay development.