Anti-RBCK1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BYM8

Core Product Specifications and Parameters

Biological Background: RBCK1 Function and Localization

• RBCK1 (RanBP-type and C3HC4-type zinc finger-containing protein 1), also known as HOIL-1, RNF54, or XAP4, is an E3 ubiquitin ligase that adopts multiple isoforms via alternative splicing.
• Functions as an E3 ubiquitin-protein ligase, accepting ubiquitin from UBE2L3/UBCM4 and transferring it to substrates such as oxidized IREB2, TAB2, and IRF3, thereby promoting proteasomal degradation. Related references: PMID:12629548 PMID:17449468 PMID:18711448
• Component of the LUBAC complex, which conjugates linear (Met‑1‑linked) polyubiquitin chains to substrates and is essential for activation of the canonical NF‑κB and JNK signaling pathways. Related references: PMID:17006537 PMID:19136968 PMID:21455173
• LUBAC‑mediated linear ubiquitination of IKBKG and RIPK1 interferes with TNF‑induced cell death, thereby preventing excessive inflammation. Related references: PMID:17006537 PMID:21455173 PMID:21455180
• In innate immunity, LUBAC decorates cytosolic bacteria with linear ubiquitin chains, serving as an 'eat‑me' signal for xenophagy and promoting NF‑κB activation. Related references: PMID:28481331
• Together with OTULIN, LUBAC regulates canonical Wnt signaling during angiogenesis. Related references: PMID:23708998
• The protein contains RanBP‑type and C3HC4‑type zinc finger domains and a coiled‑coil region; it undergoes acetylation and phosphorylation.
• Identified as an HBV‑associated factor, RBCK1 interacts with the hepatitis B virus X protein and may participate in host‑virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60–192 of human RBCK1, a region conserved across human, mouse, and rat, supporting the observed cross‑reactivity.
• For Western blot (WB), expect a band near 58 kDa; use positive control lysates from cells expressing RBCK1 (e.g., HeLa, HEK293T) and confirm specificity with knockdown controls.
• In immunofluorescence (IF/ICC), RBCK1 localizes to both cytoplasm and nucleus; optimize fixation and permeabilization for the cell type used.
• For ELISA, consider employing recombinant RBCK1 protein as a reference standard and validate assay conditions with known positive and negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one‑stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti‑RBCK1 polyclonal antibody supports investigations into ubiquitin signaling, NF‑κB pathways, and inflammatory diseases and can be procured alongside complementary products for streamlined assay development. Our sourcing capabilities also extend to rare target raw materials and technical consulting from concept to clinic.