Anti-RBBP8 Rabbit Polyclonal Antibody for WB - Q99708

Core Product Specifications and Parameters

Biological Background: DNA Endonuclease RBBP8 Function and Localization

• RBBP8, also known as CtIP, RBBP-8, RIM, or SAE2, is a DNA endonuclease that plays a critical role in DNA double-strand break repair.
• Cooperates with the MRE11-RAD50-NBN (MRN) complex to initiate DNA-end resection during homologous recombination (HR), a process restricted to S and G2 cell cycle phases (PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:23273981).
  Related references: PMID:17965729, PMID:19202191, PMID:19759395, PMID:23273981
• Acts as a key determinant of DSB repair pathway choice by committing cells to HR and inhibiting classical non-homologous end-joining (NHEJ) (PubMed:19202191).
  Related references: PMID:19202191
• Promotes the endonuclease activity of MRE11 to clear protein-DNA adducts at DSB ends, a function dependent on recruitment by NBN following CDK1-mediated phosphorylation (PubMed:27814491, PubMed:27889449, PubMed:30787182).
  Related references: PMID:27814491, PMID:27889449, PMID:30787182
• Functions downstream of ATM and the MRN complex to facilitate ATR activation and single-stranded DNA generation at DSB sites in S/G2 phase (PubMed:16581787, PubMed:17965729, PubMed:19759395).
  Related references: PMID:16581787, PMID:17965729, PMID:19759395
• Component of the BRCA1-RBBP8 complex, regulating CHEK1 activation and the G2/M checkpoint upon DNA damage (PubMed:15485915, PubMed:16818604).
  Related references: PMID:15485915, PMID:16818604
• Preferentially binds to DNA Y-junctions and blocked DNA ends, mediating intermolecular DNA bridging to facilitate repair (PubMed:30601117).
  Related references: PMID:30601117
• Localizes to the nucleus and chromosomes, and its expression is elevated in ER-positive breast cancer cells, with downregulation in ER-negative lines (at protein level).

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody targets the C-terminus (aa 648-897) of RBBP8. It is suitable for Western blot (predicted band ~102 kDa) and ELISA applications.
• Verify antibody specificity by testing against positive and negative control lysates; cross-reactivity with rat is reported, enabling rat model studies.
• For WB, consider a loading control and optimize blocking conditions to reduce non-specific binding typical of polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research institutes from concept to clinic. This anti-RBBP8 antibody aids in DNA repair and oncology investigations. For your assay development needs, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary supplies, and rare target sourcing—all through a streamlined procurement process.