Anti-RBBP6 Polyclonal Antibody for WB, ELISA - Q7Z6E9

Core Product Specifications and Parameters

Biological Background: RBBP6 Function and Localization

• Promotes ubiquitination and proteasomal degradation of YBX1 (PubMed:18851979). Also may act as a scaffold to enhance MDM2-mediated ubiquitination and degradation of p53/TP53 (By similarity). Related references: PMID:18851979
• Regulates DNA replication and chromosomal common fragile site stability by controlling ZBTB38 ubiquitination and degradation, which in turn modulates MCM10 expression (PubMed:24726359). Related references: PMID:24726359
• Isoform 1 restricts ebolavirus replication, likely by impairing the vp30-NP interaction and viral transcription.
• Contains zinc-finger domains and undergoes post-translational modifications including acetylation and phosphorylation; functions in ubiquitin conjugation via isopeptide bond formation.
• Subcellular localization includes nucleus, nucleolus, chromosomes, and cytoplasm, with association to the cytoskeleton and centrosome.
• Tissue expression: highly expressed in placenta and testis, lower in brain, heart, kidney, liver, lung; overexpressed in esophageal cancer.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment (aa 1240-1355 of human RBBP6); for mouse and rat samples, verify sequence homology with targeted regions of RBBP6 orthologs.
• For Western blotting, the predicted molecular weight is approximately 202 kDa; optimize gel percentage and transfer conditions accordingly.
• For ELISA, consider using a standard indirect ELISA protocol with appropriate secondary antibody detection; validate in the relevant sample system.
• Since RBBP6 has multiple isoforms and post-translational modifications, potential band patterns may vary; include appropriate controls and validate with known positive and negative cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for diagnostic manufacturers and researchers, covering every stage from concept to clinic. This anti-RBBP6 polyclonal antibody is available for studies on ubiquitin-mediated proteolysis and cancer biology. Our portfolio includes validated antibody pairs, optimized recombinant proteins, and ancillary reagents, with expedited sourcing for specialized targets.