Anti-RBBP4 Polyclonal Antibody for WB, ELISA, ChIP - Q09028

Core Product Specifications and Parameters

Biological Background: RBBP4 Function and Localization

• RBBP4, also known as NURF55, RBAP48, p48, is a core histone-binding subunit belonging to the WD repeat protein family.
• It is a component of the chromatin assembly factor 1 (CAF-1) complex, which facilitates nucleosome assembly on newly replicated and repaired DNA. Related references: PMID:8858152
• RBBP4 is part of multiple histone deacetylase complexes, including the core HDAC complex and the NuRD complex, promoting transcriptional repression via histone deacetylation and nucleosome remodeling. Related references: PMID:9150135 PMID:16428440 PMID:28977666 PMID:39460621
• It is also a subunit of the NURF (nucleosome remodeling factor) complex, connecting chromatin remodeling to transcriptional regulation. Related references: PMID:14609955 PMID:15310751
• As a component of the PRC2 complex, RBBP4 contributes to the repression of homeotic genes during development. Related references: PMID:29499137 PMID:31959557
• RBBP4 localizes to the nucleus, and is found at chromosome telomeres, consistent with its roles in chromatin modification.
• It is expressed in neuroblastoma cells, and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination.
• Keywords: Cell cycle, DNA damage, DNA repair, DNA replication, Transcription regulation, WD repeat.

Experimental Guidance and Technical Tips

• The recombinant full-length immunogen suggests the antibody may detect native RBBP4 protein; consider testing in relevant sample systems.
• For Western blot analysis, use reducing and denaturing conditions, and expect a band at approximately 48 kDa consistent with the protein weight.
• This antibody is reported for ChIP; appropriate crosslinking and chromatin preparation protocols should be optimized for your specific experimental system.
• ELISA can be used for quantitative detection; validate linearity and sensitivity with purified protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, supplying high-quality antibodies like this RBBP4 pAb for epigenetic and chromatin research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your entire diagnostic development pipeline. Our expert team can also assist with rare target sourcing to meet your unique project needs.