Anti-RBBP4 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q09028

Core Product Specifications and Parameters

Biological Background: RBBP4 Function and Localization

• RBBP4 (Retinoblastoma-binding protein 4) is also known as NURF55, RBAP48, and CAF-1 subunit C; it is a core histone-binding subunit that targets chromatin assembly factors, remodeling factors, and histone deacetylases to histones in a nucleosomal DNA-regulated manner (PubMed:10866654). Related references: PMID:10866654
• It is a component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair (PubMed:8858152). Related references: PMID:8858152
• RBBP4 is part of the core histone deacetylase (HDAC) complex, promoting histone deacetylation and transcriptional repression (PubMed:9150135). Related references: PMID:9150135
• It is a subunit of the NuRD (nucleosome remodeling and histone deacetylase) complex, contributing to transcriptional repression via deacetylation and nucleosome remodeling (PubMed:16428440, PubMed:28977666, PubMed:39460621). Related references: PMID:16428440 PMID:28977666 PMID:39460621
• In the PRC2 complex, RBBP4 helps repress homeotic genes during development (PubMed:29499137, PubMed:31959557). Related references: PMID:29499137 PMID:31959557
• RBBP4 is also found in the NURF (nucleosome remodeling factor) complex (PubMed:14609955, PubMed:15310751). Related references: PMID:14609955 PMID:15310751
• It localizes to the nucleus, chromosomes, and telomeres.
• The protein contains WD repeats and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination; it is expressed in neuroblastoma cells.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1–100) is conserved across human, mouse, and rat, supporting the observed cross reactivity. Confirm specificity in the target species using appropriate controls.
• For Western blotting, expect a band at approximately 48 kDa. Nuclear extracts may enhance detection sensitivity.
• IHC-P applications should include optimization of antigen retrieval and primary antibody titration to achieve robust nuclear staining.
• IF/ICC protocols should anticipate a nuclear localization pattern; include DAPI co-staining to confirm nuclear targeting.
• ELISA performance can be evaluated by direct coating of the immunogen peptide or recombinant protein; consider pairing with a validated detection antibody for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, offering validated antibodies like this RBBP4 rabbit mAb to support chromatin biology and epigenetic research. Our supply capabilities include bulk ancillary reagents, matched antibody pairs, and optimized monoclonal/polyclonal antibodies for diagnostic assay development. For rare or specialized targets, we leverage extensive sourcing networks to provide timely access to critical raw materials, helping you streamline your workflow from concept to clinic.